Journal: Medicine

Article Title: Immune checkpoint proteins PD-1 and TIM-3 are both highly expressed in liver tissues and correlate with their gene polymorphisms in patients with HBV-related hepatocellular carcinoma

doi: 10.1097/MD.0000000000005749

Figure Lengend Snippet: PD-1 expression in liver tissues (×200). (A) Tumor tissues of HCC; (B) tumor adjacent tissues of HCC; (C) liver tissues of cirrhosis; (D) IHC score of PD-1 expression in (a) tumor tissues of HCC, (b) tumor adjacent tissues of HCC, and (c) liver tissues of cirrhosis. Black arrows indicate examples of positive staining cells. HCC = hepatocellular carcinoma, IHC = immunohistochemistry, PD-1 = programmed death-1.

Article Snippet: The sections were incubated with primary antibodies mouse monoclonal anti-PD-1 antibody (Abcam, Cambridge, MA) at a dilution of 1:50 or rabbit polyclonal anti-TIM-3 antibody (BioVision, Milpitas, CA) at a dilution of 1:150 at 37 °C for 30 minutes.

Techniques: Expressing, Staining, Immunohistochemistry

Journal: Medicine

Article Title: Immune checkpoint proteins PD-1 and TIM-3 are both highly expressed in liver tissues and correlate with their gene polymorphisms in patients with HBV-related hepatocellular carcinoma

doi: 10.1097/MD.0000000000005749

Figure Lengend Snippet: PD-1 expression in tumor tissues of HBV-related HCC (n = 171), tumor adjacent tissues of HCC (AT, n = 171), and cirrhotic liver tissues (LC, n = 34) according to the genotypes of PD1 polymorphism. AA, AG, and GG = genotype AA, genotype AG, and genotype GG of PD1 +8669 G/A (rs10204525) polymorphism, HBV = hepatitis B virus, HCC = hepatocellular carcinoma, IHC = immunohistochemistry, AT = adjacent tissues of HCC, LC = liver cirrhosis, PD-1 = programmed death-1.

Article Snippet: The sections were incubated with primary antibodies mouse monoclonal anti-PD-1 antibody (Abcam, Cambridge, MA) at a dilution of 1:50 or rabbit polyclonal anti-TIM-3 antibody (BioVision, Milpitas, CA) at a dilution of 1:150 at 37 °C for 30 minutes.

Techniques: Expressing, Immunohistochemistry

Journal: Oncology Letters

Article Title: Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma

doi: 10.3892/ol.2014.2356

Figure Lengend Snippet: Representative immunohistochemical streptavidin-peroxidase staining in extranodal natural killer/T-cell lymphoma (upper row) and rhinitis tissues (lower row). The positive cases of (A and D) programmed death 1, (B and E) PD-L1 and (C and F) PD-L2 (magnification, ×200). PD-L, programmed death ligand.

Article Snippet: The antigen retrieval was conducted in 0.01 mol/l citrate (pH 6.0) and the slides were incubated overnight with rabbit anti-human PD-L1 polyclonal antibody (1:120; Proteintech, Chicago, IL, USA), rabbit anti-human PD-L2 polyclonal antibody (1:150) and mouse anti-human PD-1 monoclonal antibody (mAb; 1:100) (both ZSGB-BIO, Beijing, China) and phosphate-buffered saline was used as a blank control.

Techniques: Immunohistochemical staining, Staining

Journal: Oncology Letters

Article Title: Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma

doi: 10.3892/ol.2014.2356

Figure Lengend Snippet: PD-1 expression in (A) CD4 + and (B) CD8 + T-cell subsets in 20 ENKL patients was significantly increased compared with that in 10 HVs (P<0.05). Representative PD-1 expression in (C) CD4 + and (D) CD8 + T-cell subsets in six ENKL patients was (E) downregulated with chemotherapy. (F) T-helper cell type 1 cytokine (IL-2 and IFN-γ) mean production levels in the serum of 20 ENKL patients were significantly lower than those in 10 HVs (P<0.05). PD1, programmed death 1; ENKL, extranodal natural killer/T-cell lymphoma; HVs, healthy volunteers; IL-2 interleukin 2; IFN-γ, interferon γ.

Article Snippet: The antigen retrieval was conducted in 0.01 mol/l citrate (pH 6.0) and the slides were incubated overnight with rabbit anti-human PD-L1 polyclonal antibody (1:120; Proteintech, Chicago, IL, USA), rabbit anti-human PD-L2 polyclonal antibody (1:150) and mouse anti-human PD-1 monoclonal antibody (mAb; 1:100) (both ZSGB-BIO, Beijing, China) and phosphate-buffered saline was used as a blank control.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma

doi: 10.3892/ol.2014.2356

Figure Lengend Snippet: (A) Purity of CD8 + T cells separated by magnetic-activated cell sorting was 99%. (B) Purity of CD8 + PD-1 + T cells was 96.2% following the stimulation of allogeneic CD8 + T cells with phytohemagglutinin for 48 h. (C) SNK-6 cells were used as the control group and, following the coculture of SNK-6 cells and CD8 + T cells for 72 h, a significant inhibitory effect of PD-L1 on allogeneic CD8 + T-helper type 1 cytokine (IL-2 and IFN-γ) secretion was observed; (A and B) P<0.05. (D) CD8 + T-cell apoptosis in groups A and B was not altered significantly compared with activated CD8 + T cells at 72 h (P>0.05). (E) SNK-6 cells were used as the control group and cells harvested at 0, 24, 48 and 72 h were analyzed by flow cytometry gating CFSE + events. The proliferation index was not significantly different among the groups (P>0.05). PD-1, programme death 1; PD-L. programmed death ligand; IL-2 interleukin 2; IFN-γ, interferon γ; CFSE, carboxy-fluorescein succinimidyl ester.

Article Snippet: The antigen retrieval was conducted in 0.01 mol/l citrate (pH 6.0) and the slides were incubated overnight with rabbit anti-human PD-L1 polyclonal antibody (1:120; Proteintech, Chicago, IL, USA), rabbit anti-human PD-L2 polyclonal antibody (1:150) and mouse anti-human PD-1 monoclonal antibody (mAb; 1:100) (both ZSGB-BIO, Beijing, China) and phosphate-buffered saline was used as a blank control.

Techniques: FACS, Flow Cytometry